The PB smear review is a qualitative examination of the blood smear to detect clinically significant abnormalities in all cells, including leukocytes, erythrocytes, and platelets.7 A slide review is appropriate in all patients with unexplained leukocytosis, which requires proper slide preparation and staining. Blood smears are prepared from fresh, anticoagulated blood or from a drop of fresh blood from a syringe or finger-stick puncture. Blood films may be prepared manually using slides or coverslips or by instruments. A properly prepared smear is wedge shaped with a slightly rounded edge and shows a normal gradation of blood from thick to thin. The edges of the smear should not touch the edge of the slide because this area must be available for scanning. After air drying, the slides should be stained with Romanowsky-type stains such as May-Grünwald-Giemsa and Wright stains; DNA and RNA are labeled a deep basophilic color, the nuclei blue-purple, and the cytoplasm pink, depending on cytoplasmic granule composition. The area between cells should be clean and free of precipitate.
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